Immunophenotyping of patients and heterozygous relatives. (A) Representative flow cytometry dots plots showing increased levels of DNTs in P3 and normal levels in healthy control, heterozygous relative, and P2. (B) Representative flow cytometry dot plots showing normal levels of Tregs (CD3⁺CD4⁺CD25⁺CD127^low) in patients and heterozygous relative, lack of Tfh cells (CD3⁺CD4⁺CXCR5⁺CD45RO⁺), reduced Th1 cells (CD3⁺CD4⁺CCR6⁻CXCR3⁺) and Th17 cells (CD3⁺CD4⁺CCR6⁺CXCR3⁻), and differentiation preference toward Th2 cells (CD3⁺CD4⁺CCR6⁻CXCR3⁻) in P2 and P3.