Snai1 overexpression perturbs myeloid lineage development. (A) Immunophenotypic characterization of hematopoietic stem (LSK) and myeloid progenitor (MP) cell populations within the bone marrow of Snai1 transgenic mice. (B) Flow cytometric analysis showing representative dot plots of LSK gated cells. (C) Within the stem cell compartment, there was a significant increase in the number of short-term HSCs (ST-HSCs; LIN⁻cKIT⁺SCA1⁺CD34⁺CD135⁻) but no change in the number of long-term HSCs (LT-HSCs; lin⁻cKit⁺Sca1⁺CD34⁻-CD135⁻) or multipotent progenitors (MPPs; LIN⁻cKIT⁺SCA1⁺CD34⁺CD135⁺). (D) Representative dot plots of LT-HSC, ST-HSC, and MPP gated cells are shown. (E) A significant increase in the number of common myeloid progenitors (CMPs; LIN⁻cKIT⁺SCA1⁻CD34⁺CD16/32⁻) GMPs (LIN⁻cKIT⁺SCA1⁻CD34⁺CD16/32⁺) was observed; however, the megakaryocyte/erythroid progenitor (MEP; LIN⁻cKIT⁺SCA1⁻CD34⁻CD16/32⁻) population was slightly reduced (significant only in the heterozygous mice). (F) Representative FACs plots of myeloid progenitor gating are shown. (G) A significant increase in the number of immature (CD11b⁺Ly6G^lo) and mature myeloid cells (monocytes CD11b⁺GR1⁻ and granulocytes CD11b⁺Ly6G^hi) was also evident in the bone marrow of Snai1 transgenic mice. (H) Flow cytometric analysis showing representative dot plots of myeloid gated cells. (A,C,E,G) Data are represented as mean + standard error of the mean for Snai1^+/+ (n = 13), Snai1^tg/+ (n = 8), and Snai1^tg/tg (n = 7) biological replicates.*P < .05, **P < .01, ***P < .001 Student 2-sided unpaired t test.