Red blood cell (RBC) processing and workflow. Whole blood in EDTA was centrifuged to fractionate the blood into components. The plasma and white blood cell layers are removed, leaving the RBC layer. Hemoglobin was then removed with successive washes of hypotonic phosphate-buffered saline until a white erythrocyte pellet remained. The glycoproteins were extracted with a selective detergent using a commercially available kit. Glycoprotein fractions were interrogated by lectin microarray and analyzed with unbiased and predictive data models.