Cellular correlates of aGVHD onset. (A) Ratios of the frequency of CD3⁺ T cells within lymphocytes (left panel), CD4⁺ T cells within CD3⁺ T cells (middle panel), and CD8⁺ T cells within CD3⁺ T cells (right panel) between recipients and donors (R/D) in aGVHD vs No aGVHD couples. (B) Ratios of the frequency of T_Naive, T_SCM-like, T_CM, T_EM, and T_EMRA cells within CD4⁺ cells (upper panels) and CD8⁺ cells (lower panels) between R/D in aGVHD vs No aGVHD couples. Horizontal lines indicate the median. For this analysis, samples from the 2 cohorts were pooled. All recipients without aGVHD or with grade ≥2 aGVHD were included in the analysis (No GVHD, n = 36; GVHD, n = 22). P values were calculated using the Mann-Whitney U test; differences are considered significant for P < .05.