Dynamics of 14-3-3ζ and c-Src binding to integrin-β3 and the inhibiting effect of myr-KF7 and THO. (A-B) Washed human platelets (1000 × 109/L) were preincubated with myr-KF7 or myr-KF7scr at 50 μM, THO at 100 μM, SQ29548 at 5 μM, and apyrase at 1 U/mL in the presence of 100 μg/mL fibrinogen, and the platelets were activated by 1 μM anti-αIIb TM. Platelet lysates were harvested in 30 seconds and immunoprecipitated with integrin-β3 antibody, and the immunoprecipitates were blotted with integrin-β3, c-Src pY416, c-Src, and 14-3-3ζ antibodies. (C-D) Washed human platelets (1000 × 109/L), preincubated with or without 100 μM THO, were activated by thrombin (0.03 U/mL). Then, the lysates were harvested at various time points, immunoprecipitated with integrin-β3 antibody and blotted with integrin-β3, Src pTyr416, c-Src, and 14-3-3ζ antibodies. All data are expressed as means ± SD (n = 3). Statistical significance was determined by Student t test. *P < .05; **P < .01.