Effects of myr-KF7 and THO on platelet c-Src, RhoA, and AKT activation. (A-B) Washed human platelets pretreated with 50 μM myr-KF7, myr-KF7scr, THO, and 0.1% DMSO were allowed to adhere to immobilized fibrinogen and then solubilized at the indicated time points. Proteins from lysates were immunoblotted with antibodies to Src pY416 and c-Src. (C-D) GTP-bound RhoA was measured by association with the RhoA-binding domain of Rhotekin (GST-RBD). Washed human platelets (500 × 109/L) pretreated with myr-KF7 (E-F) or THO (G-H) were activated by 0.03 U/mL thrombin or 1 μg/mL collagen, and the lysates were immunoblotted with AKT1 pS473 or AKT1 antibody. (I-J) Platelets pretreated with myr-KF7 or THO were activated by anti-αIIb TM peptide (1 μM), and the lysates were immunoblotted with AKT1 pS473 or AKT1 antibody. All data are expressed as means ± SD (n = 3). Statistical significance was determined by Student t test. *P < .05; **P < .01.