Figure 2.
Interference with the 14-3-3ζ–c-Src–integrin-β3 complex by KF7 and THO. (A-D) SPR analysis of the interaction between KF7 or THO and 14-3-3ζ immobilized on Sensor Chip CM-5 and EL17 immobilized on Sensor Chip NTA. SPR sensorgrams of KF7 binding to 14-3-3ζ (A) and EL17 (B) or THO binding to 14-3-3ζ (C) and EL17 (D). (E) Western blot analysis of recombinant c-Src or 14-3-3ζ protein precipitated by biotin-conjugated β3CT in the presence of 0.1% DMSO, 30 μM KF7, 30 μM KF7 scrambled peptide (KF7scr), and 30 μM THO. (F) Western blot analysis of recombinant c-Src protein precipitated by biotin-conjugated WT β3CT in the presence of 100 μM KF7, KF7scr, THO or 0.1% DMSO. Data are means ± SD (n = 3). Statistical significance was determined by Student t test. **P < .01.

Interference with the 14-3-3ζ–c-Src–integrin-β3 complex by KF7 and THO. (A-D) SPR analysis of the interaction between KF7 or THO and 14-3-3ζ immobilized on Sensor Chip CM-5 and EL17 immobilized on Sensor Chip NTA. SPR sensorgrams of KF7 binding to 14-3-3ζ (A) and EL17 (B) or THO binding to 14-3-3ζ (C) and EL17 (D). (E) Western blot analysis of recombinant c-Src or 14-3-3ζ protein precipitated by biotin-conjugated β3CT in the presence of 0.1% DMSO, 30 μM KF7, 30 μM KF7 scrambled peptide (KF7scr), and 30 μM THO. (F) Western blot analysis of recombinant c-Src protein precipitated by biotin-conjugated WT β3CT in the presence of 100 μM KF7, KF7scr, THO or 0.1% DMSO. Data are means ± SD (n = 3). Statistical significance was determined by Student t test. **P < .01.

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