![Comparative analysis of tomo-seq data sets identifies species-restricted and interspecies-conserved gene expression in the aortic HSC supportive microenvironment. (A) Venn diagram showing shared and distinct ventrally expressed genes between chicken, human, mouse, and zebrafish tomo-seq data sets. Genes in bold: upregulated (messenger RNA level) in HSPC supportive stromal cell line UG26.1B6 compared with the non-/less supportive stromal cell line UG26.3B5 isolated from the embryonic AGM (according to http://stemniche.snv.jussieu.fr/29); underlined: known HSPC regulators in the embryonic aorta. Italic denotes connected to hematopoiesis. *Connected to leukemia. (B-F) Tomo-seq traces for PODXL (blue), ALDH1A2 (green), and PPARGC1A (orange) in the (ventral) aortic region (R1) of an E3 chicken slice (B), a 35-day-old human slice (C), an E10.5 mouse slice (D), a 28-hpf whole zebrafish (E), and a 40-hpf zebrafish trunk (F). (G-J) ISH showing the specific expression patterns of PODXL, ALDH1A2, and PPARGC1A in the (ventral) aortic region (R1) of an E3 chicken anterior slice (G), a 35-day-old human slice (H), an E10.5 mouse slice (I), a 40-hpf whole zebrafish embryo (J, left panel), and a zebrafish trunk slice (J, right panel). The number of embryos analyzed for WISH is indicated on the pictures. (K-L) Ten of the top biological process GO terms (K) and most relevant KEGG pathways (L) enriched in at least 2 species tomo-seq data sets (ie, 2, 3, and 4 species data sets). Bars represent 150 μm (G,I-J [left panels]), 200 μm (H), and 50 μm (J [right panels]).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/136/7/10.1182_blood.2019004446/3/bloodbld2019004446f2.png?Expires=1722746298&Signature=HzFunyE~lSMNjP-NVpkCEE8AEvMc8Bkb-039D-6ZoUY49AsdYfABnGO6NkqVjTWahDha42X4QwDxeFJKUSCDtCpz~izfudcNpNnO-ZVJKSiU~NoVUfPH0Igtea3FLTpYX4XWIRH9TbEs~teP-Psg6hYWx1l8~-mhenF-GwaDLUJx~inRgEm8TjG~R~ogDamUFGMlvYjDTrsJx41983XMUpdWL6m4FkyAujxLmgzQakwb3U~KXO33HAx-tX~gggvD87MEqjgIMdoXrm78y86wqtmOdZ~TfXNXtce2cFqm6rcKQt7WhFAFHGbPJB9pFqVWnAfdp8-YRzoWVI0pHC7-KA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Comparative analysis of tomo-seq data sets identifies species-restricted and interspecies-conserved gene expression in the aortic HSC supportive microenvironment. (A) Venn diagram showing shared and distinct ventrally expressed genes between chicken, human, mouse, and zebrafish tomo-seq data sets. Genes in bold: upregulated (messenger RNA level) in HSPC supportive stromal cell line UG26.1B6 compared with the non-/less supportive stromal cell line UG26.3B5 isolated from the embryonic AGM (according to http://stemniche.snv.jussieu.fr/29 ); underlined: known HSPC regulators in the embryonic aorta. Italic denotes connected to hematopoiesis. *Connected to leukemia. (B-F) Tomo-seq traces for PODXL (blue), ALDH1A2 (green), and PPARGC1A (orange) in the (ventral) aortic region (R1) of an E3 chicken slice (B), a 35-day-old human slice (C), an E10.5 mouse slice (D), a 28-hpf whole zebrafish (E), and a 40-hpf zebrafish trunk (F). (G-J) ISH showing the specific expression patterns of PODXL, ALDH1A2, and PPARGC1A in the (ventral) aortic region (R1) of an E3 chicken anterior slice (G), a 35-day-old human slice (H), an E10.5 mouse slice (I), a 40-hpf whole zebrafish embryo (J, left panel), and a zebrafish trunk slice (J, right panel). The number of embryos analyzed for WISH is indicated on the pictures. (K-L) Ten of the top biological process GO terms (K) and most relevant KEGG pathways (L) enriched in at least 2 species tomo-seq data sets (ie, 2, 3, and 4 species data sets). Bars represent 150 μm (G,I-J [left panels]), 200 μm (H), and 50 μm (J [right panels]).