Determining opsonophagocytic response induced by 4CMenB vaccination. (A) Whole blood-mediated killing of MenB using PNH patient plasma from before and after the first and second 4CMenB vaccinations and immune plasma from healthy individuals. Figure shows boxplots with minimum/maximum whiskers; horizontal lines and values indicated in the figure are medians. Statistical analyses were performed with Prism version 5.03 for Windows. Repeated measures analysis of variance with Tukey’s posttest was used to determine statistical significance. No statistically significant differences were found. The filled circles represent killing of MenB with plasma from patient 1 and patient 54. Complement survival of MenB in HI plasma and normal plasma taken before and after the first and second 4CMenB vaccinations: Patient 1 (B) and Patient 54 (C).