Neutrophils use moesin to strengthen adhesion under flow. (A) Rolling velocities of neutrophils of the indicated genotype on P-selectin with or without coimmobilized ICAM-1 in the presence or absence of anti-ICAM-1 mAb. (B) Percentages of neutrophils of the indicated genotype rolling, arrested and round, or arrested and spread on coimmobilized P-selectin, ICAM-1, and CXCL1. The surface areas of arrested cells are quantified in supplemental Figure 4. (C) Percentages of round or spread neutrophils of the indicated genotype adherent to immobilized anti-β2 integrin F(ab′)2. The surface areas of adherent cells are quantified in supplemental Figure 5. (D-E) Bone marrow leukocytes of the indicated genotype were allowed to accumulate on the indicated substrate at 0.5 dyne/cm2 for 5 minutes, and then wall shear stress was increased every 30 seconds. The percentage of remaining adherent cells was measured. The data in panels A-E represent the mean ± SEM from 5 experiments, with 5 mice in each experimental group. *P < .05.