Neutrophil signaling through PSGL-1 requires moesin to activate NETs but not to generate deep vein thrombi. (A) Percentage of NET-forming neutrophils from the indicated genotype, stimulated with P-selectin-IgM or phorbol myristate acetate (PMA), calculated by dividing the number of cells with both extracellular citrullinated histones and Sytox Orange-positive DNA by the total number of Sytox-Orange positive cells. (B) Thrombus frequency (left panel) and thrombus weight (right panel) 24 hours after ligation of the inferior vena cava in mice of the indicated genotype. The data represent the mean ± SEM from 10 to 15 mice in each group. *P < .05.