Neutrophils lacking ERMs polarize and redistribute raft-associated proteins to uropods. (A) Bone marrow neutrophils from control or DKO mice were fixed and labeled with CTxB to label GM1 (green) or with antibodies to the indicated protein (red). Representative cells were visualized by phase-contrast microscopy or confocal microscopy. Scale bar, 5 μm. (B) Neutrophils from control or DKO mice were stimulated with CXCL1, fixed, labeled, and visualized as in panel A. The data in panels A and B are representative of 3 experiments. (C) Percentage of vehicle- or CXCL1-treated neutrophils with capped GM1 (recognized by CTxB), PSGL-1, or CD44 visualized by confocal microscopy. The data represent the mean ± standard deviation from 3 experiments. *P < .05.