Characterization of ERM-deficient neutrophils. (A) Western blot of bone marrow neutrophils from the indicated genotype, probed with antibodies that recognize all 3 ERMs equivalently (top row), ezrin only (second row), moesin only (third row), or the control protein β-actin (bottom row). The data are representative of 3 experiments. Quantitative data are presented in supplemental Figure 1. (B) Leukocyte and platelet numbers from peripheral blood of control or DKO mice. The data represent the mean ± SEM from 6 to 9 experiments. (C) Left and middle panels, scanning electron micrographs of peripheral blood neutrophils from control and DKO mice. The boxed areas are magnified in the insets. Scale bar, 1 μm. Right panel, quantification of microvilli. The data represent the mean ± SEM from 32 to 35 neutrophils from 4 mice of each genotype. (D) Flow cytometric analysis of neutrophils from peripheral blood or bone marrow from the indicated genotype, probed with antibodies to the indicated protein. The data are representative of 5 experiments. Quantification of the data as mean fluorescence intensity is presented in supplemental Figure 2. (E) Flow cytometric analysis of L-selectin expression on bone marrow neutrophils of the indicated genotype 15 minutes after incubation with buffer or WKYMVm peptide. The data represent the mean ± standard deviation from 3 experiments. (F) Flow cytometric analysis of phalloidin binding to polymerized actin in permeabilized bone marrow neutrophils of the indicated genotype at the indicated times after stimulation with WKYMVn peptide. The data represent the mean ± SEM from 5 mice per genotype. *P < .05. MFI, mean fluorescence intensity.