BH3 profiling predicts sensitivity to BH3 mimetics. (A) Spearman correlations of the IC₅₀ of venetoclax, navitoclax, and AZD5991 with either protein abundance, gene expression by RNAseq, or functional dependence assessed by BH3 profiling. Cell lines: n = 21. (B) Receiver operating characteristic curve of the IC₅₀ values of AZD5991 in cell lines with >0.25 vs ≤0.25 relative cytochrome c release upon treatment with 10 µM MS1. (C) Correlation of dependence on BCL2, BCL2L1 (BCL-xL), or MCL1 defined by relative cytochrome c release by BH3 profiling and the correlating dependency score resulting from a genome-wide CRISPR Cas9 screen (ZMAD score).