A characterization of the BCL2 family in cell line models of TCL. (A) Immunoblot of BAX, BAK, BIM, BCL2, BCL-xL, MCL1, NOXA, and α-Tubulin. (B) RNA expression levels by reads per kilobase of transcript, per million mapped reads, corresponding to panel A. (C) Copy number variants by whole exome sequencing, corresponding to panels A and B. (D) BH3 profiling corresponding to panels A-C using BIM at 10, 1, 0.3, and 0.1 µM; BAD and HRK at 80 µM; and MS1 at 10 µM. The heatmap illustrates mean values of the relative cytochrome c release in ≥3 independent replicates per cell line. (E) IC₅₀ values of venetoclax (ABT-199), navitoclax (ABT-263), and AZD5991 at 72 hours corresponding to panels A-D. Bar graphs indicate mean values of at least 2 independent experiments in quadruplicates, error bars indicate the standard error of the mean.