Figure 6.
Analysis of endothelial Src kinase in the OvCa-Chip. (A) Confocal micrographs showing the status of endothelial cell adhesion junction VE-cadherin (green), expression of Src kinase (red), and its activation (phosphorylated, yellow) in the Control-Chip, OvCa-Chip, and Drug-OvCa-Chip. Bar represents 25 µm. Nuclei are stained with 4′,6-diamidino-2-phenylindole (blue). Fluorescence-activated cell sorting analysis of immunostained endothelial cells showing VE-cadherin, Src expression, and Src phosphorylation (B), and phosphorylation analysis of endothelial Src kinase by Luminex assay (C), obtained from the Control-Chip, OvCa-Chip, and Drug-OvCa-Chip. Error bars are the mean ± SEM. One-way ANOVA, followed by Dunnett’s multiple-comparisons test; n = 3 individual experiments. *P < .05.

Analysis of endothelial Src kinase in the OvCa-Chip. (A) Confocal micrographs showing the status of endothelial cell adhesion junction VE-cadherin (green), expression of Src kinase (red), and its activation (phosphorylated, yellow) in the Control-Chip, OvCa-Chip, and Drug-OvCa-Chip. Bar represents 25 µm. Nuclei are stained with 4′,6-diamidino-2-phenylindole (blue). Fluorescence-activated cell sorting analysis of immunostained endothelial cells showing VE-cadherin, Src expression, and Src phosphorylation (B), and phosphorylation analysis of endothelial Src kinase by Luminex assay (C), obtained from the Control-Chip, OvCa-Chip, and Drug-OvCa-Chip. Error bars are the mean ± SEM. One-way ANOVA, followed by Dunnett’s multiple-comparisons test; n = 3 individual experiments. *P < .05.

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