T-cell markers associated with T-cell phenotype, functionality, and response to fungi. (A) Diagram describing gating strategy and t-SNE mapping and analysis workflow for samples shown in B and C. t-SNE plot showing marker expression levels for single parameters (B, activation markers and cytokines; C, phenotype markers) on individual cells of panfungal T-cell products cultured for 6 hours with nonpulsed DCs or fungus-pulsed DCs. Responding fungus-specific T cells correspond to the cell cluster showing higher GM-CSF and TNF-α expression. t-SNE plots are representative of control and pulsed DC-treated panfungal T cells from 3 experiments.