How the vascular gap is closed after a neutrophil has crossed. A neutrophil leaves the circulation by penetrating in between two individual endothelial cells (ECs). Consequently, it leaves behind a gap in the endothelial lining. Platelets stick to von Willebrand factor, release angiopoeitin-1 (Ang1), resulting in activating the endothelial receptor Tie2. Inset: Tie2 activation triggers intracellular endothelial signals, including the actin remodelling proteins RhoGEF FGD5 and small RhoGTPase Cdc42, resulting in lateral protrusions and closing of the gap.