Evaluation of HSCs and bone features in newborn th3 mice. (A) Frequency of HSCs on BM Lin⁻ cells from WT and th3 mice at different ages. WT, n ≥ 5 mice per group; th3, n ≥ 5 mice per group. (B) Analysis of cell cycle distribution among G0/G1, S, and G2/M phases by cytofluorimetry after 7-AAD staining of HSCs from newborn WT and th3 mice. WT, n = 7; th3, n = 7. (C) Total BMD of tibiae from newborn mice analyzed by pQCT. WT, n = 9; th3, n = 6. (D) Evaluation of PTH levels in sera from newborn mice. WT, n = 3; th3, n = 3. **P < .01