MKs with Myh9-RD mutations have aberrant distribution in BM. (A) Diagram depicting the domains of NMIIA molecule with MYH9-RD mutations highlighted in red. DAPI, 4′,6-diamidino-2-phenylindole. (B-G) Confocal images of femoral cryosections from Myh9-RD mice models and WT littermates following immunostaining for sinusoids (laminin) and MK (CD41). Yellow tracings represent the junction of bone and BM. Scale bar, 100 μm. (H) Scatterplots representing median distances of MKs from the endosteal niche (WT^GFP+/−, n = 938; R702C^GFP+/−, n = 777; WT, n = 1236; D1424N^+/−, n = 1513; WT, n = 1101; E1841K^+/−m N = 1165). Images were acquired with a Plan-Fluor ×63 objective (numerical aperture [NA], 1.4) of a Zeiss LSM 780 confocal microscope with Zeiss AxioCam. Images were processed and stitched together with ZEN software from Zeiss. (I-K) Ploidy levels in Myh9-RD mutant MKs harvested from mouse BM analyzed by flow cytometry, represented as mean ± standard error of the mean from ≥3 mice of each genotype.