Inhibition of AURKB and SAC in CD34⁺ HSPCs reproduces chromosome structure defects and hyperdiploid karyotypes. (A) Schematic depicting the workflow for AURKB and SAC inhibition in CD34⁺ HSPCs. (B) Representative images of metaphase chromosomes treated as indicated. (C-F) Frequency of metaphases with micronuclei (n = 500 cells per experiment) (C), hypocondensed chromosomes (D), PCS (E), and hyperdiploidy karyotype (F). (G) Representative FACS analysis showing PI staining profiles in CD34⁺ HSPCs treated as indicated; n = 150 metaphases analyzed per treatment from 3 independent experiments. *P < .05, **P < .01 (1-tailed Student t test).