Figure 3.
The loss of the HECT domain of Ubr5 leads to reduction in marginal and B1a splenic cells. (A) Bar graph of the total number of splenocytes (N ≥ 4). (B) Bar graph of frequency of B220+ cells per spleen (N = 7). (C) Representative flow cytometry plots gated on B220+ for follicular B cells (B220+CD21+CD23+) and marginal zone B cells (B220+CD21+CD23−) (N = 7). (D-E) Bar graphs of percentages and absolute number of follicular and marginal zone B cells within the spleen. (F) Representative flow cytometry plots gated for B1a (B220+CD19loCD5+) and B1b cells (B220+CD19loCD5−) (N ≥ 8). (G-H) Bar graph of percentages and absolute number of B1a and B1b cells. *P < .05, **P < .01, ***P < .001, ****P < .0001.

The loss of the HECT domain of Ubr5 leads to reduction in marginal and B1a splenic cells. (A) Bar graph of the total number of splenocytes (N ≥ 4). (B) Bar graph of frequency of B220+ cells per spleen (N = 7). (C) Representative flow cytometry plots gated on B220+ for follicular B cells (B220+CD21+CD23+) and marginal zone B cells (B220+CD21+CD23−) (N = 7). (D-E) Bar graphs of percentages and absolute number of follicular and marginal zone B cells within the spleen. (F) Representative flow cytometry plots gated for B1a (B220+CD19loCD5+) and B1b cells (B220+CD19loCD5−) (N ≥ 8). (G-H) Bar graph of percentages and absolute number of B1a and B1b cells. *P < .05, **P < .01, ***P < .001, ****P < .0001.

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