ETS1 directly regulates expression of several key transcription factors linked to NK cell differentiation. (A) NK cells were sorted from d18 control and ETS1 p27 cultures, and RNA-sequencing was performed. Volcano plot showing downregulated (blue) and upregulated (red) genes in NK cells from ETS1 p27 vs control (n = 4). Differentially expressed NK cell–related transcription factors are indicated. (B-C) NK cell–linked transcription factors that were differentially expressed as shown in panel A were evaluated by quantitative polymerase chain reaction analysis in all stages. Data are presented as mean ± SEM, and the number of experiments is indicated. (D) Representative dot plots of flow cytometry analysis of TBET and EOMES expression in mature NK cells. The mean fluorescence intensity (MFI) ± SEM (n = 8) is shown. Significant difference compared with control, *P < .05 and ***P < .001 (Student t test). mNK, mature NK cells.