Smad and Stat signaling in LPS-treated mice with variable liver iron content. Liver protein extracts and RNA from mice described in Figure 6 were used for western blotting and qPCR analysis, respectively. (A) Levels of pStat3, Stat3, pSmad5, Smad5, TfR1, and ferritin; (B) Socs3 mRNA; (C) Inhbb mRNA; (D) Bmp6 mRNA; (E) Id1 mRNA; (F) Smad7 mRNA; (G) (Fpn + IRE) mRNA (IRE-containing isoform); (H) (Fpn − IRE) mRNA (isoform lacking IRE). All data are presented as the mean plus or minus SEM. Dotted lines indicate average values obtained from age-matched male C57BL/6 mice (n = 3) on standard diet. Statistical analysis was performed by 1-way ANOVA. Statistically significant differences (P < .05) across treatments (vs columns a, b, c) are indicated by a, b, c.