Livers of Hjv-deficient mice retain inflammatory Smad signaling, presumably via activin B. (A-E) Liver protein extracts and RNA from mice described in Figure 2 were used for western blotting and qPCR analysis, respectively. (A) Levels of pStat3, Stat3, pSmad5, and Smad5; (B) Socs3 mRNA; (C) Inhbb mRNA; (D) Bmp6 mRNA; (E) Id1 mRNA. All data are presented as the mean plus or minus SEM. Statistical analysis was performed by 2-way ANOVA. Statistically significant differences (P < .05) across genotypes (vs columns a, a′, b′) are indicated by a, a′, b′, and across LPS treatment by pound sign (#).