Figure 2.
IL27Ra−HSCs and IL27Ra−/− HSCs possess higher self-renewal capacity. (A,C) The percentage of indicated donor-derived cells (overall, myeloid cell, B cell, T cell) in secondary recipients at indicated timepoints (see Methods). (A) N = 8 mice per group, (C) N = 5-6 mice per group; data are shown as mean ± SEM. The gating strategy to generate these line plots are presented in supplemental Figure 1K. (B,D) The lineage distribution of indicated donor-derived peripheral blood cells of secondary recipients at the fourth month. (B) N = 8 mice per group, (D) N = 5-6 mice per group; data are shown as mean ± SEM. The gating strategy to generate these line plots are presented in supplemental Figure 1L. (E) Five 23- to 28-month-old IL27Ra−/− and 6 age-matched WT mice were analyzed for HSCs. This histogram depicts the number of HSCs (CD34− CD150+ KSL), lymphoid-biased HSCs (CD34− CD150middle KSL), and myeloid-biased HSCs (CD34− CD150high KSL) per million bone marrow cells. Data are shown as mean ± SEM. (F) Seven 20- 24-month-old IL27Ra−/− and 8 age-matched WT mice were analyzed for myeloid, B, and T cells. This histogram depicts the percentage of myeloid, B, and T cells in peripheral blood. Data are shown as mean ± SEM. (G-J) Freshly isolated 50 HSCs from either (G-H) 2-month-old IL27Ra−/− mice and age-matched WT mice or (I-J) 18-month-old IL27Ra−/− mice and age-matched WT mice were transplanted into lethally irradiated recipients together with 2 × 105 competitor cells. Three months after transplantation, 2 million bone marrow cells of primary recipients were transplanted into secondary recipients. Chimera in peripheral blood was checked every month until the third month. (G,I) These line plots depict the percentage of donor cell reconstitution in overall (CD45.2+), myeloid (Mac-1+), B (B220+), and T (CD3+) cell every month after transplantation. (H,J) This histogram displays the lineage distribution of donor-derived peripheral blood at the third month. (G-H) N = 5 mice per group, (I-J) N = 5-7 mice per group; data are shown as mean ± SEM.

IL27RaHSCs and IL27Ra−/− HSCs possess higher self-renewal capacity. (A,C) The percentage of indicated donor-derived cells (overall, myeloid cell, B cell, T cell) in secondary recipients at indicated timepoints (see Methods). (A) N = 8 mice per group, (C) N = 5-6 mice per group; data are shown as mean ± SEM. The gating strategy to generate these line plots are presented in supplemental Figure 1K. (B,D) The lineage distribution of indicated donor-derived peripheral blood cells of secondary recipients at the fourth month. (B) N = 8 mice per group, (D) N = 5-6 mice per group; data are shown as mean ± SEM. The gating strategy to generate these line plots are presented in supplemental Figure 1L. (E) Five 23- to 28-month-old IL27Ra−/− and 6 age-matched WT mice were analyzed for HSCs. This histogram depicts the number of HSCs (CD34 CD150+ KSL), lymphoid-biased HSCs (CD34 CD150middle KSL), and myeloid-biased HSCs (CD34 CD150high KSL) per million bone marrow cells. Data are shown as mean ± SEM. (F) Seven 20- 24-month-old IL27Ra−/− and 8 age-matched WT mice were analyzed for myeloid, B, and T cells. This histogram depicts the percentage of myeloid, B, and T cells in peripheral blood. Data are shown as mean ± SEM. (G-J) Freshly isolated 50 HSCs from either (G-H) 2-month-old IL27Ra−/− mice and age-matched WT mice or (I-J) 18-month-old IL27Ra−/− mice and age-matched WT mice were transplanted into lethally irradiated recipients together with 2 × 105 competitor cells. Three months after transplantation, 2 million bone marrow cells of primary recipients were transplanted into secondary recipients. Chimera in peripheral blood was checked every month until the third month. (G,I) These line plots depict the percentage of donor cell reconstitution in overall (CD45.2+), myeloid (Mac-1+), B (B220+), and T (CD3+) cell every month after transplantation. (H,J) This histogram displays the lineage distribution of donor-derived peripheral blood at the third month. (G-H) N = 5 mice per group, (I-J) N = 5-7 mice per group; data are shown as mean ± SEM.

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