Molecular characterization of neutrophil differentiation in normal and tumor BM. (A) Unsupervised clustering after RNAseq of immature, intermediate, and mature neutrophils from HAs and MM patients (n = 8 each) showed accurate segregation per cell type and not participant. (B) Whole-transcriptome profiling through RNAseq segregates mature neutrophils from HAs and MM patients according to 108 genes differentially expressed (P < .05). (C) Gene set enrichment analysis showed that mature granulocytes from MM patients increased activation of pathways related to inflammation and reduced antiviral and anticancer type 1 and 2 interferon transcriptional response. (D) Mature neutrophils from HAs (n = 3) were treated with TGF-β, and expression levels of the top-10 differentially expressed genes between MM and HA neutrophils (panel B) were analyzed. There were no significant differences when we compared mature neutrophils from MM patients vs HAs treated with TGF-β (P > .05). CND, condition; ns, not significant; TNFA, tumor necrosis factor α.