Peptide 617F (YGVCFCGDENI) facilitates the binding of AGK and JAK2. (A) Coimmunoprecipitation of the lysates of HEK293T cells transfected with AGK-Flag and JAK2-HA/JAK2-V617F-HA using HA beads or Flag beads. (B) Immunoprecipitation of the lysates of platelet from WT mice and JAK2V617F mice with anti-AGK or anti-JAK2 antibodies. (C) Sequences of peptides 617V and 617F. (D) Coimmunoprecipitation of the lysates of HEK293T cells transfected with AGK-Flag and JAK2-HA and incubated with or without peptide 617F/617V using HA beads or Flag beads. IB, immunoblot.