Characterization of fibrinogen, D98, and D-dimer under reducing and nonreducing conditions by Coomassie blue staining and immunoblot analysis with antibodies specific for the fibrinogen β- and γ-chains. The locations of the intact α-, β-, and γ-chains of fibrinogen (Fib) are indicated in the Coomassie blue–stained reduced sample. The immunoblot of reduced D-dimer (DD) indicates that virtually all of the γ-chains in D-dimer are in the form of γ-γ dimers.