Serial dilution of IDH1- and IDH2-mutated cells with IDH1/IDH2 wild-type DNA.IDH1 and IDH2 samples were mutated at a VAF of 50%. Mutated DNA was diluted with DNA from IDH1/IDH2 wild-type cells up to 30 000-fold, and 8 different dilutions were tested in 3 independent replicates. RF approach indicates error-corrected sequencing analysis. R1/R2 indicates analysis by forward/reverse read correction.