Analysis of EPO target genes in the BM and spleen of Tfr2^BMKO/Hbb^th3/+mice. The expression levels of EPO target genes were determined in BM cells (A-C) and spleen samples (D-F) from Hbb^th3/+ and Tfr2^BMKO/Hbb^th3/+ mice 9 and 22 weeks after BMT. In the figure are graphed: messenger RNA (mRNA) expression of ERFE (Erfe, A,D), B-cell lymphoma–extra large (Bcl-x_L; B,E), and Fas ligand (Fasl; C,F) relative to glyceraldehyde 3-phosphate dehydrogenase (Gapdh). Bars indicate SE. Asterisks refer to statistically significant differences between age-matched Hbb^th3/+ and Tfr2^BMKO/Hbb^th3/+ mice: *P < .05; **P < .01. Ct, cycle threshold.