ICA decreases GVHD-associated inflammatory cytokines in allo-BMT recipients. (A-F) Lethally irradiated B10.BR recipients were transplanted with TCD-BM in combination with purified T cells from C57Bl/6 mice to induce GVHD. Mice received daily oral gavage of 150 mg/kg ICA. Samples were collected at day 21 posttransplant (n = 5 mice per condition, assayed in triplicate). Representative data from 1 of 3 experimental repeats. Cytokines (A) and chemokines (B) significantly altered in colon homogenates in mice transplanted with T-cell-depleted BM + T and following treatment with ICA (day 21). (C) Cytokines significantly altered in plasma in mice transplanted with T-cell-depleted BM + T and following treatment with ICA (day 21). (D) IL-22 in colon (left) and plasma (right) in mice transplanted with T-cell-depleted BM + T and following treatment with ICA (day 21). (E) Percentages of CD69 and PD1 positive CD4 donor T cells (left) or CD8 donor T cells (right) in mice treated with ICA or VEH. (F) Percentages of cytokine expressing CD4 donor T cells (left) or CD8 donor T cells (right) in mice treated with ICA or VEH. Statistics: Mann-Whitney rank sum. Error bars represent standard deviations. *P = .01 to .05; NS, not significant. Mantel Cox log-rank (survival curve).