Monocytes give rise to RPMs in the spleen during the recovery from anemia induced by PHZ. (A) Cx3cr1^{YFPCreER;tdTomato} mice were IP injected with tamoxifen in corn oil at 75 mg/kg body weight consecutively for 5 days prior to single PHZ injection at 100 mg/kg. n = 4 for each group per time point. At 24, 48, 72, and 96 hours after PHZ treatment, flow cytometry analysis was done to characterize monocytes, pre-RPMs, and RPMs in YFP⁺tdTomato^neg cells, YFP⁺tdTomato⁺ cells, and YFP^negtdTomato⁺ cells in whole spleens and Ter119⁺ EBIs. (B-C) Representative flow cytometry diagrams illustrating phenotypic dynamics of monocytes, pre-RPMs, and RPMs in (B) whole spleens and (C) enriched Ter119⁺EBIs. (D) Proportion of F4/80⁺Vcam-1⁺ Ly6C^loCD11b^lo RPMs (left). The percentage of Ly6C^loCD11b^lo RPMs that are F4/80⁺Vcam1⁺ (right) in the whole spleen. (E) Proportion of F4/80⁺Vcam-1⁺ Ly6C^loCD11b^lo RPMs (left) and the percentage of Ly6C^loCD11b^lo RPMs that are F4/80⁺Vcam1⁺ (right) in Ter119⁺EBIs.