Nuclear FOXO1 induces proliferation and survival in mouse BL cell lines. (A) Growth curves of mouse BL cell lines and their isogenic Foxo1 KO clones. Cells characterized by a heterozygous T24 mutation (mouse BL #82) or cell lines exclusively expressing wt FOXO1 (mouse BL#81 and #88) were analyzed. Individual clones of the parental cell lines (CT) and FOXO1-ablated clones (KO) were analyzed over time. The graph summarizes data of 3 experiments. Bars indicate the standard deviation. ****P < .0001 (Wilcoxon–Mann-Whitney test). (B) Representative FACS analysis of 5-bromo-2′-deoxyuridine (BrdU) and 7-aminoactinomycin D (7-AAD) staining in parental and Foxo1 KO cells. For each cell line, a single clone per condition (CT and KO) is shown. The gating strategy for dead (R1) and living (R2) cells is depicted. Numbers indicate the gate frequency (in percent). (C) Quantification of dead cells (R1 in panel B) and proliferating cells (G0/G1; S; G2/M in panel B) in cell line clones as analyzed in panel A. Bars indicate the standard deviation. *P < .05; **P < .01; ***P < .001; ****P < .0001 (Wilcoxon–Mann-Whitney test). ns, not significant.