Figure 6.
Loss of 1 copy of Abi-1 accelerates disease development in the bone marrow transplantation model of MPLW515L-mediated myeloproliferative neoplasm. (A) Schematic representation of Abi-1HET/MPLW515L model. Bone marrow was obtained from 14-week-old sex-matched Abi-1WT (n = 3) or Abi-1HET (n = 3) animals and transduced with retrovirus encoding Abi-1WT or W515L mutated GFP-tagged MPL. Infected bone marrows representing 4 experimental groups Abi-1WT/MPLWT, Abi-1HET/MPLWT, Abi-1WT/MPLW515L, or Abi-1HET/MPLW515L were next transplanted to C57BL/6J females (n = 6 per experimental group). Analyses were conducted 60 days posttransplant. (B). Representative gross pathology images of femurs, spleens, and livers of the animals from each experimental group are shown. (C) Average spleen and liver sizes of Abi-1WT/MPLWT, Abi-1HET/MPLWT, Abi-1WT/MPLW515L, or Abi-1HET/MPLW515L mice. Relative organ weight was calculated as an absolute organ weight (g)/body weight on sacrifice day (g) ×100. Organs from 6 animals per group were evaluated. (D) Average white blood cell count and hematocrit and platelet count of Abi-1WT/MPLWT, Abi-1HET/MPLWT, Abi-1WT/MPLW515L, or Abi-1HET/MPLW515L mice. Peripheral blood from 6 animals per group was analyzed. (E) Average frequencies of Mac1+/Gr-1+, CD41+, CD42+, CD71+/Ter119+ or CD71+/Ter119− as well as B220+ and CD3+ cells in the bone marrow or peripheral blood obtained from Abi-1WT/MPLWT, Abi-1HET/MPLWT, Abi-1WT/MPLW515L, or Abi-1HET/MPLW515L mice. Samples from 6 animals per group were analyzed. (F) Gomori reticulin staining of the bone marrow from femurs and spleen sections of Abi-1WT/MPLW515L or Abi-1HET/MPLW515L animals (black stain). Bars correspond to 100 μm. Images were obtained using Zeiss Axiophot microscope with Zeiss Pan-Apochromat 20×/1.0 lens. *P < .05

Loss of 1 copy of Abi-1 accelerates disease development in the bone marrow transplantation model of MPLW515L-mediated myeloproliferative neoplasm. (A) Schematic representation of Abi-1HET/MPLW515L model. Bone marrow was obtained from 14-week-old sex-matched Abi-1WT (n = 3) or Abi-1HET (n = 3) animals and transduced with retrovirus encoding Abi-1WT or W515L mutated GFP-tagged MPL. Infected bone marrows representing 4 experimental groups Abi-1WT/MPLWT, Abi-1HET/MPLWT, Abi-1WT/MPLW515L, or Abi-1HET/MPLW515L were next transplanted to C57BL/6J females (n = 6 per experimental group). Analyses were conducted 60 days posttransplant. (B). Representative gross pathology images of femurs, spleens, and livers of the animals from each experimental group are shown. (C) Average spleen and liver sizes of Abi-1WT/MPLWT, Abi-1HET/MPLWT, Abi-1WT/MPLW515L, or Abi-1HET/MPLW515L mice. Relative organ weight was calculated as an absolute organ weight (g)/body weight on sacrifice day (g) ×100. Organs from 6 animals per group were evaluated. (D) Average white blood cell count and hematocrit and platelet count of Abi-1WT/MPLWT, Abi-1HET/MPLWT, Abi-1WT/MPLW515L, or Abi-1HET/MPLW515L mice. Peripheral blood from 6 animals per group was analyzed. (E) Average frequencies of Mac1+/Gr-1+, CD41+, CD42+, CD71+/Ter119+ or CD71+/Ter119 as well as B220+ and CD3+ cells in the bone marrow or peripheral blood obtained from Abi-1WT/MPLWT, Abi-1HET/MPLWT, Abi-1WT/MPLW515L, or Abi-1HET/MPLW515L mice. Samples from 6 animals per group were analyzed. (F) Gomori reticulin staining of the bone marrow from femurs and spleen sections of Abi-1WT/MPLW515L or Abi-1HET/MPLW515L animals (black stain). Bars correspond to 100 μm. Images were obtained using Zeiss Axiophot microscope with Zeiss Pan-Apochromat 20×/1.0 lens. *P < .05

Close Modal

or Create an Account

Close Modal
Close Modal