Figure 6.
Altered DNA methylation explains a minority of gene-expression changes after Dnmt3a loss. (A) Venn diagram showing overlap of gene significantly hypomethylated between compared data sets.24,26,43 (B) Venn diagram showing overlap between the hypomethylated gene core and the DNMT3A gene-expression core. (C) GSEA plot representing PRC2 complex regulated genes in edited LSK from Jak2VF-Dnmt3a-Cas9 and Jak2VF-Cas9 recipient mice. (D-G) GSEA plot representing PRC2 complex (EED, EZH2, and SUZ12) in edited LSK from Jak2VF-Dnmt3a-Cas9 and Jak2VF-Cas9 recipient mice.