Myelofibrotic transformation in Jak2^VF-Dnmt3a-Cas9 is associated with HSC depletion and the accumulation of MPPs. (A) Representative gating strategy used to identify HSPCs (LK, CMP, GMP, MEP, LSK, MPP, ST-HSC, LT-HSC) based on expression of GFP, lineage expression markers (Lin), c-Kit, Sca-1, CD34, FCγRIIIA, CD48, and CD150 in BM. (B) Percentage of LT-HSC, (C) ST-HSC, (D) MPP, (E) GMP, (F) CMP, and (G) MEP in GFP⁺ and GFP⁻ compartment from BM of Jak2^VF-Cas9 and Jak2^VF-Dnmt3a-Cas9 LSK⁺ recipient mice (week 32). Absolute numbers of (H) LT-HSC, (I) ST-HSC, (J) MPP, (K) GMP, (L) CMP, and (M) MEP in GFP⁺ and GFP⁻ compartment from BM of Jak2^VF -Cas9 and Jak2^VF-Dnmt3a-Cas9 LSK recipient mice (week 32), including Cas9⁻ unedited controls. In all figures, P values were calculated using the unpaired Student t test, comparing Jak2^VF-Dnmt3a-Cas9 to Jak2^VF-Cas9, *P < .05, **P < .01, ***P < .001 with mean ± SEM.