Morphology of myelofibrotic transformation in bone marrow and spleen. (A) Representative image of BM of WT Dnmt3a-Cas9 (left), Jak2^VF-Cas9 (center), and Jak2^VF-Dnmt3a-Cas9 (right) LSK-transplanted mice at week 32. Scale bar, 50 µm; H&E stain. (B) Representative image of reticulin staining from BM of WT, Jak2^VF-Cas9, and Jak2^VF-Dnmt3a-Cas9 LSK-transplanted mice. Scale bar, 50 µm. Insets, Enlarged image. (C) Representative image of spleen of WT Dnmt3a-Cas9, Jak2^VF-Cas9, and Jak2^VF-Dnmt3a-Cas9 LSK-transplanted mice. Scale bar, 50 µm. (D) Representative image of reticulin staining from spleen of WT Dnmt3a-Cas9, Jak2^VF-Cas9, and Jak2^VF-Dnmt3a-Cas9 LSK-transplanted mice. Scale bar, 50 µm. (E) Spleen weight (left panel) and image (right panel) from Jak2^VF-Cas9 and Jak2^VF-Dnmt3a-Cas9 LSK-transplanted mice at week 32. P values were calculated using the unpaired Student t test, ***P < .001 mean ± SEM. (F) BM cell count from Jak2^VF-Cas9 and Jak2^VF-Dnmt3a-Cas9 LSK-transplanted mice. P values were calculated using the unpaired Student t test, ***P < .001 mean ± SEM. (G) Peripheral blood from WT Dnmt3a-Cas9, Jak2^VF-Cas9, and Jak2^VF-Dnmt3a-Cas9 LSK-transplanted mice. Scale bar, 50 µm; Wright-Giemsa stain.