Neutrophil recruitment is reduced in Tln1^3mutmice. (A-D) In vivo leukocyte rolling velocity, adhesion efficiency, and extravasation were analyzed in mouse cremaster muscle venules of WT and Tln1^3mut mice 2 hours after intrascrotal injection of TNF-α. Rolling velocity (A) and adhesion efficiency (B) (adherent cells per mm² normalized to the total neutrophil count) were analyzed using intravital microscopy. (C) Leukocyte extravasation was assessed in the perivascular region upon Giemsa staining of cremaster muscle whole mounts (n = 17 vessels in 4 WT and 4 Tln^3mut mice). Values are given as means ± standard errors of the mean. (D) Representative images of Giemsa-stained whole mounts of WT and Tln1^3mut mice. Arrows point to extravasated neutrophils. Scale bar, 30 µm. *P < .05, ***P < .001.