TET2 knockdown led to the SCF-dependent clonal expansion of CFU-E cells. (A) Growth of sorted single luciferase or TET2-knockdown CFU-E cells cultured in the presence of EPO for 7 days. (B) Growth of sorted single luciferase or TET2-knockdown CFU-E cells cultured in the presence of EPO plus SCF for 15 days. Note that cells can be divided into HP and LP populations. (C) Representative cytospin images of erythroblasts from panel B. (D) mRNA levels of AXL in the LP cells (pooled) and HP cells (pooled). (E) Western blot analysis of AXL of pooled LP and HP cells. (F) Quantitative analysis of AXL protein levels from 3 independent experiments. *P < .05. NS, not statistically significant.