Effects of TET2 knockdown on DNA methylation. (A) DNA dot blots for 5mC. (B) DNA dot blots for 5hmC. (C) Quantitative analysis of 5mC and 5hmC levels from 3 independent experiments. (D) AXL promoter region containing 6 CCGG sites (blue). (E-F) Methylation-sensitive restriction enzyme analysis of 5mC and 5hmC changes at −414, +320 CCGG sites of AXL promoter. *P < .05.