Upregulation of AXL in marker CFU-E cells. (A) Number of differentially expressed genes among luciferase CFU-E, TET2-knockdown CFU-E, and TET2-knockdown marker CFU-E cells, as revealed by RNA-seq analysis. Fold change > 2. (B) Heat map of the 6 genes involved in the “organ regeneration” pathway. (C) mRNA levels of AXL, as revealed by RNA-seq. (D) mRNA levels of AXL, as assessed by qRT-PCR. (E) Western blot analysis of AXL. (F) Quantitative analysis of AXL protein levels from 3 independent experiments. (G) Growth curves of sorted luciferase and TET2-knockdown CFU-E cells in the presence of DMSO or 0.2 μM R428. (H) Flow cytometry analysis showing GPA expression of luciferase and TET2-knockdown CFU-E cells cultured for 13 days in the presence of DMSO or 0.2 μM R428. (I) Flow cytometry analysis showing band 3/α4 integrin expression of luciferase and TET2-knockdown CFU-E cells cultured for 13 days in the presence of DMSO or 0.2 μM R428. (J) Quantitative analysis of results shown in panel I. (K) Representative cytospin images of erythroblasts. *P < .05, **P < .01, ***P < .001.