TET2 knockdown impaired human terminal erythroid differentiation. (A) Representative flow cytometry analysis of GPA expression of sorted luciferase and TET2-knockdown CFU-E cells cultured in the presence of EPO plus SCF for different days, as indicated. (B) Quantitative analysis of changes in GPA⁻ population from 3 independent experiments. (C) Terminal erythroid differentiation was monitored on the indicated days by flow cytometric analysis based on the expression of band 3 and α4 integrin. Representative plots of α4-integrin vs band 3 of GPA⁺ cells are shown, and the erythroblasts are separated into 5 populations: proerythroblasts (Pro; α4-integrin^hiband 3^neg), early basophilic erythroblasts (EB; α4-integrin^hiband 3^low), late basophilic erythroblasts (LB; α4-integrin^hiband 3^med), polychromatic erythroblasts (Poly; α4-integrin^medband 3^med), and orthochromatic erythroblasts (Ortho; α4-integrin^lowband 3^hi). (D) Quantitative analyses of results shown in panel C from 3 independent experiments. (E) Representative cytospin images of erythroblasts cultured for different days as indicated. *P < .05, **P < .01.