Hyperproliferation of TET2-knockdown CFU-E cells in the presence of SCF. (A) qRT‐PCR analyses of TET2 mRNA levels (normalized to actin) of sorted luciferase and TET2-knockdown erythroid cells. (B) Colony-forming ability of sorted luciferase and TET2-knockdown CFU-E cells in the presence of EPO only (left panel) or in the presence of EPO plus SCF (right panel). Scale bar, 50 μm. (C) Percentage of larger-size colonies in panel B. (D) Growth curves of sorted luciferase and TET2-knockdown CFU-E cells cultured in the presence of EPO plus SCF. (E) Numbers of cell divisions of sorted erythroblasts at the indicated stages in culture. All results are from 3 independent experiments. *P < .05, **P < .01, ***P < .001.