American Society of Hematology

Figure 2.

Biophysical properties of the PolyHb used in this study. (A) O2 equilibrium curves for PolyHb and Hb with 3 runs per sample. (B) Comparison of the time course for deoxygenation in the presence of 1.5 mg/mL sodium dithionite for oxygenated Hb and PolyHb. For deoxygenation, the reactions were monitored at 437.5 nm and 20°C in 0.1 M pH 7.4 phosphate-buffered saline. (C) Time courses for the NO dioxygenation reaction with oxygenated Hb and PolyHb at NO 12.5 μM and 25 μM. Dots represent experimental data, and the corresponding solid lines of the same color represent curve fits to the data. NO dioxygenation reactions were monitored at 420.0 nm and 20°C in 0.1 M pH 7.4 phosphate-buffered saline. (D) Comparison of the NO dioxygenation rates for Hb and PolyHb. For kinetics, the data show an average of 10 kinetic traces for each sample. The error bars indicate the standard deviation from 10 replicates. (E) Representative intensity distributions of the hydrodynamic diameter of PolyHb. (F) Summary of the biophysical properties of unmodified Hb and PolyHb.

Biophysical properties of the PolyHb used in this study. (A) O₂ equilibrium curves for PolyHb and Hb with 3 runs per sample. (B) Comparison of the time course for deoxygenation in the presence of 1.5 mg/mL sodium dithionite for oxygenated Hb and PolyHb. For deoxygenation, the reactions were monitored at 437.5 nm and 20°C in 0.1 M pH 7.4 phosphate-buffered saline. (C) Time courses for the NO dioxygenation reaction with oxygenated Hb and PolyHb at NO 12.5 μM and 25 μM. Dots represent experimental data, and the corresponding solid lines of the same color represent curve fits to the data. NO dioxygenation reactions were monitored at 420.0 nm and 20°C in 0.1 M pH 7.4 phosphate-buffered saline. (D) Comparison of the NO dioxygenation rates for Hb and PolyHb. For kinetics, the data show an average of 10 kinetic traces for each sample. The error bars indicate the standard deviation from 10 replicates. (E) Representative intensity distributions of the hydrodynamic diameter of PolyHb. (F) Summary of the biophysical properties of unmodified Hb and PolyHb.

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