The TM G412D gene expression (RT-PCR products) and protein production in the patient. (A) The expression of TM G412D mRNA in the PBMCs of the patient (Pt) was detected by RT-PCR and was comparable with that in PBMCs of a healthy control (WT). NC, negative control. (B) The TM G412D protein concentration in the patient’s plasma was found to be 17.5 ng/ml using a pAb-ELISA, but was not detected by mAB-ELISA, recognizing the thrombin-binding site (MFTM-4).