Ldb1 and Lyl1 control expression of a similar cohort of genes that are dysregulated in Lmo2-tg thymocytes. (A-B) Deletion of Ldb1 or Lyl1 has similar effects on gene expression in Lmo2-tg DN thymocytes. Shown are the expression of genes that are upregulated (A) or downregulated (B) in Lmo2-tg thymocytes and that are normalized by deletion of either Lyl1³⁷  or Ldb1. (C-D) Deletion of Ldb1 normalizes expression of genes reported to be upregulated (C) or downregulated (D) in human ETP-ALL.^5,38  Results shown are from RNA-seq of DN2/3 thymocytes from B6, Lmo2-tg, or Rag1-Cre;Ldb1^fl/fl;Lmo2-tg mice. For each of the genes shown in panels A-D, normalization of gene expression (ie, trending toward that of wt (B6) in Rag1-Cre;Ldb1^fl/fl;Lmo2-tg thymocytes compared with Lmo2-tg was statistically significant (P < .05).