MKL2 knockdown in fibroblasts. (A) Knockdown of MKL2 in primary fibroblasts upon shRNA lentiviral transduction was assessed by western blotting. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as loading control. (B) Quantification of MKL2 levels in panel A. (C) Confocal analysis of fibroblasts by staining for F-actin (green, F-actin [phalloidin]; cyan, DNA [Hoechst]). Images were acquired with a Leica TCS SP8 confocal microscope. Scale bars, 100 µm. SCR, scrambled.