Intratumoral heterogeneity in MF. Combined data from SVs and CNAs for each sample underwent phylogenetic analysis to identify genetic subclones, as in Figure 1. (A) Rainbow graph representing the number and frequency of the subclones identified in each sample. Samples are arranged by an increasing number of subclones. Top bar graph shows TCF for each sample; color of the bar indicates type of lesion (ESP, LSP, tumor [TMR]). (B) Examples of 3 major categories of phylogenetic trees: nonbranched linear sequence of subclones (upper), simple branched structure with 1 generation of subclones (middle), and complex structure with several generations of subclones (lower). All phylogenetic trees are shown in supplemental Figure 3. (C) Bubble plot showing correlation between the number of neoplastic clonotypes and number of subclones in the samples. Size of the bubble is proportional to the frequency of the first-ranked (most abundant) clonotype. Dashed line highlights the samples where the first-ranked clonotype had a relative frequency of ≥60%.