Figure 2.
Immunohistochemical analysis of MF engrafted NSG and MISTRG mice BM. (A) Number of human megakaryocytes (Mega, CD61+) per 4 high power fields (HPF, equaling 1 mm2) in NSG and MISTRG mice for patient (PT) samples with ≥10% hCD45+ (grand median, unpaired Student t test). (B) Representative IHC analysis for hCD61 and Gömöri (stains reticulin fibers) of the BM of an untransplanted MISTRG mouse, a MISTRG mouse transplanted with a healthy donor sample (HD), a NSG and a MISTRG mouse transplanted with sample from a post–essential thrombocythemia patient (PT 12), and the BM biopsy from the same patient (scale bars, 50 µm; original magnification ×400). Arrows point to atypical human MF megakaryocytes. Arrowheads point to atypical human ET megakaryocytes. ns, not significant.

Immunohistochemical analysis of MF engrafted NSG and MISTRG mice BM. (A) Number of human megakaryocytes (Mega, CD61+) per 4 high power fields (HPF, equaling 1 mm2) in NSG and MISTRG mice for patient (PT) samples with ≥10% hCD45+ (grand median, unpaired Student t test). (B) Representative IHC analysis for hCD61 and Gömöri (stains reticulin fibers) of the BM of an untransplanted MISTRG mouse, a MISTRG mouse transplanted with a healthy donor sample (HD), a NSG and a MISTRG mouse transplanted with sample from a post–essential thrombocythemia patient (PT 12), and the BM biopsy from the same patient (scale bars, 50 µm; original magnification ×400). Arrows point to atypical human MF megakaryocytes. Arrowheads point to atypical human ET megakaryocytes. ns, not significant.

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